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vglut2 ires cre transgenic mice  (Jackson Laboratory)

 
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    Jackson Laboratory vglut2 ires cre transgenic mice
    Vglut2 Ires Cre Transgenic Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vglut2+ires+cre+mice/cre+vglut2/pm42251246-31-16-25
    Average 86 stars, based on 1 article reviews
    vglut2 ires cre transgenic mice - by Bioz Stars, 2026-09
    86/100 stars

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    Article Title: Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice
    Article Snippet: The following mouse lines were used in this study: wild-type C57BL/6 (SLAC Laboratory Animal, Shanghai), Vglut2 -ires-Cre mice (The Jackson Laboratory, Stock 016963), Vgat -ires-Cre mice (The Jackson Laboratory, Stock 016962).

    Article Title: Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice.
    Article Snippet: The following mouse lines were used in this study: wild-type C57BL/6 (SLAC laboratory Animal, Shanghai), Vglut2-ires-Cre mice (The Jackson Laboratory, Stock 016963), Vgat-ires-Cre mice (The Jackson Laboratory, Stock 016962).

    Article Title: EphB1-NR2B receptor signaling in glutamatergic neurons of the ventroposteromedial thalamic nucleus regulates emergence from anesthesia
    Article Snippet: Male C57BL/6J mice and Vglut2-ires-cre mice (the Jackson Laboratory, 016963), aged 6 to 10 weeks, were used in this study.

    Article Title: EphB1-NR2B receptor signaling in glutamatergic neurons of the ventroposteromedial thalamic nucleus regulates emergence from anesthesia.
    Article Snippet: Male C57BL/6J mice and Vglut2- ires- cre mice (the Jackson Laboratory, 016963), aged 6 to 10 weeks, were used in this study.

    Mutagenesis:

    Article Title: VTA monosynaptic connections by local glutamate and GABA neurons and their distinct roles in behavior.
    Article Snippet: .. vglut2-IRES-Cre mice (JAX # 016963, The Jackson Laboratories, Bar Harbor, ME; on a mixed C57BL/6;FVB;129S6 genetic background) and slc32a1-IRES2-FlpO-D mice (vgat-FlpO-D, Jax # 031331, The Jackson Nature Communications | (2025) 16:8500 15 Laboratories, on a mixed 129S6/SvEvTac × C57BL/6NCrl genetic background, deposited42) were crossed to produce the male and female vglut2-Cre/ vgat-Flp mice (20–30 g) used in this study. vglut2IRES-Cre and th-2A-Flpomice (C57BL/6N-Thtm1Awar/Mmmh, in C57BL/ 6 J background from the Mutant Mouse Resource and Research Centers, Davis, CA) were crossed to produce the vglut2-Cre/th-Flp male and female mice (20–30 g) used in this study. vglut2-Cre mice (Slc17a6tm2(cre)Lowl/J, in C57BL/6 J background from The Jackson Laboratories) and vgat-Cre mice (Slc32a1tm2(cre)Lowl/J, in C57BL/6 J background fromThe JacksonLaboratories)werebred in theNIDA/IRP animal facility (20–30 g) and were used in photoinhibition experiments. ..

    Mouse Assay:

    Article Title: Low-dimensional population dynamics in the brainstem gate REM sleep.
    Article Snippet: .. Experiments were performed in GAD2-IRES-Cre mice ( Jackson Laboratory stock no. 010802), C57BL/6 mice ( Jackson Laboratory stock no. 000664) and VGLUT2-IRES-Cre mice ( Jackson Laboratory stock no. 016963). ..



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    The excitatory versus inhibitory effects of VTA GG input depends on postsynaptic membrane potential in LHb. (A) Strategy to express ChR2:eYFP in VTA neurons positive for both <t>VGLUT2-Cre</t> and VGAT-Flp (VTA GG ). (B) ChR2 expression in VTA, scale bar = 250 um. (C) Terminal expression of ChR2 in LHb, scale bar = 1 mm. (D) Optogenetically-evoked inhibitory postsynaptic currents (oIPSCs; purple) were observed in LHb neurons in response to 50ms blue light pulses at holding voltage (V h ) = 0 mV and blocked by picrotoxin (PTX; paired t-test: t 3 =3.4, p=0.04), whereas optogenetically-evoked excitatory postsynaptic currents (oEPSCs; green) were observed at V h =-60 mV and sensitive to 6,7-dinitroquinoxaline-2,3-dione (DNQX; paired t-test: t 4 =2.8, p=0.04); scale: x= 50 ms, y=20 pA. (E) Most recorded neurons displayed a GABA/AMPA ratio greater than 1 (one-sample t-test vs. ratio of 1: t 14 =4.9, p=0.0002). (F) In current-clamp mode (I=0), optogenetic-evoked inhibitory postsynaptic potentials (oIPSPs, purple) and-excitatory postsynaptic potentials (oEPSPs, green) were observed in different LHb cells; scale: x=50 ms, y=5 mV. (G) The sign and scale of oPSP displayed as a function of resting membrane potential of postsynaptic LHb cell (Vm; Pearson r =-0.65, p=0.005), and (H) neurons in which terminal stimulation of VTA GG inputs caused an oEPSP were more hyperpolarized compared to cells that responded with an oIPSP (Unpaired t-test: t 15 =2.9, p=0.01). (I) Example trace of a LHb neuron given hyperpolarizing and depolarizing current injections, with blue light pulse delivered halfway through 1s current step; scale: x=100 ms, y=20 mV. (J) Mean oPSPs were positive during negative current steps, and negative during positive current steps (One-way ANOVA: F 6,82 =18.8, p<0.0001). (K) oPSPs plotted by membrane voltage at each corresponding current step (Pearson R correlation: r=-0.71, p<0.0001). Data are shown as mean +/-SEM and/or individual points; *p<0.05, **p<0.01, ****p<0.0001.
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    The excitatory versus inhibitory effects of VTA GG input depends on postsynaptic membrane potential in LHb. (A) Strategy to express ChR2:eYFP in VTA neurons positive for both VGLUT2-Cre and VGAT-Flp (VTA GG ). (B) ChR2 expression in VTA, scale bar = 250 um. (C) Terminal expression of ChR2 in LHb, scale bar = 1 mm. (D) Optogenetically-evoked inhibitory postsynaptic currents (oIPSCs; purple) were observed in LHb neurons in response to 50ms blue light pulses at holding voltage (V h ) = 0 mV and blocked by picrotoxin (PTX; paired t-test: t 3 =3.4, p=0.04), whereas optogenetically-evoked excitatory postsynaptic currents (oEPSCs; green) were observed at V h =-60 mV and sensitive to 6,7-dinitroquinoxaline-2,3-dione (DNQX; paired t-test: t 4 =2.8, p=0.04); scale: x= 50 ms, y=20 pA. (E) Most recorded neurons displayed a GABA/AMPA ratio greater than 1 (one-sample t-test vs. ratio of 1: t 14 =4.9, p=0.0002). (F) In current-clamp mode (I=0), optogenetic-evoked inhibitory postsynaptic potentials (oIPSPs, purple) and-excitatory postsynaptic potentials (oEPSPs, green) were observed in different LHb cells; scale: x=50 ms, y=5 mV. (G) The sign and scale of oPSP displayed as a function of resting membrane potential of postsynaptic LHb cell (Vm; Pearson r =-0.65, p=0.005), and (H) neurons in which terminal stimulation of VTA GG inputs caused an oEPSP were more hyperpolarized compared to cells that responded with an oIPSP (Unpaired t-test: t 15 =2.9, p=0.01). (I) Example trace of a LHb neuron given hyperpolarizing and depolarizing current injections, with blue light pulse delivered halfway through 1s current step; scale: x=100 ms, y=20 mV. (J) Mean oPSPs were positive during negative current steps, and negative during positive current steps (One-way ANOVA: F 6,82 =18.8, p<0.0001). (K) oPSPs plotted by membrane voltage at each corresponding current step (Pearson R correlation: r=-0.71, p<0.0001). Data are shown as mean +/-SEM and/or individual points; *p<0.05, **p<0.01, ****p<0.0001.

    Journal: bioRxiv

    Article Title: GABA-glutamate corelease is a mechanism for state-dependent neurotransmission

    doi: 10.1101/2025.11.19.689286

    Figure Lengend Snippet: The excitatory versus inhibitory effects of VTA GG input depends on postsynaptic membrane potential in LHb. (A) Strategy to express ChR2:eYFP in VTA neurons positive for both VGLUT2-Cre and VGAT-Flp (VTA GG ). (B) ChR2 expression in VTA, scale bar = 250 um. (C) Terminal expression of ChR2 in LHb, scale bar = 1 mm. (D) Optogenetically-evoked inhibitory postsynaptic currents (oIPSCs; purple) were observed in LHb neurons in response to 50ms blue light pulses at holding voltage (V h ) = 0 mV and blocked by picrotoxin (PTX; paired t-test: t 3 =3.4, p=0.04), whereas optogenetically-evoked excitatory postsynaptic currents (oEPSCs; green) were observed at V h =-60 mV and sensitive to 6,7-dinitroquinoxaline-2,3-dione (DNQX; paired t-test: t 4 =2.8, p=0.04); scale: x= 50 ms, y=20 pA. (E) Most recorded neurons displayed a GABA/AMPA ratio greater than 1 (one-sample t-test vs. ratio of 1: t 14 =4.9, p=0.0002). (F) In current-clamp mode (I=0), optogenetic-evoked inhibitory postsynaptic potentials (oIPSPs, purple) and-excitatory postsynaptic potentials (oEPSPs, green) were observed in different LHb cells; scale: x=50 ms, y=5 mV. (G) The sign and scale of oPSP displayed as a function of resting membrane potential of postsynaptic LHb cell (Vm; Pearson r =-0.65, p=0.005), and (H) neurons in which terminal stimulation of VTA GG inputs caused an oEPSP were more hyperpolarized compared to cells that responded with an oIPSP (Unpaired t-test: t 15 =2.9, p=0.01). (I) Example trace of a LHb neuron given hyperpolarizing and depolarizing current injections, with blue light pulse delivered halfway through 1s current step; scale: x=100 ms, y=20 mV. (J) Mean oPSPs were positive during negative current steps, and negative during positive current steps (One-way ANOVA: F 6,82 =18.8, p<0.0001). (K) oPSPs plotted by membrane voltage at each corresponding current step (Pearson R correlation: r=-0.71, p<0.0001). Data are shown as mean +/-SEM and/or individual points; *p<0.05, **p<0.01, ****p<0.0001.

    Article Snippet: Slc17a6 +/Cre (VGLUT2-IRES-cre) mice and Slc32a1 Flp+/- (VGAT-Flp) mice were initially obtained from Jackson Laboratory (Stocks: 018147 and 029591, respectively), and were crossed to generate a dual transgenic, VGLUT2-cre/VGAT-Flp line.

    Techniques: Membrane, Expressing

    The reinforcing effects of VTA GG input to LHb require GABA release. (A) Strategy to express ChR2:eYFP in VTA GG neurons, plus optic fibers implanted bilaterally above LHb. (B) Terminal expression of ChR2 in LHb of VGLUT2-Cre; VGAT-Flp mice and (C) control mouse lacking Flp; scale = 500 um. (D) Schematic of optogenetic self-stimulation assay. Nose pokes into the active port delivered 40 Hz 1s blue (473 nm) light plus a 1s tone, while nose pokes into the inactive port delivered a 1s tone alone. (E) ChR2-expressing mice (Flp+; n=10) nose poked at greater amounts in the active port compared to the inactive port (Two-Way ANOVA, main effect of port-type: F 1,9 =29, p=0.0004; day x port interaction: F 2,18 =9.3, p=0.002; Bonferroni multiple comparisons between active vs. inactive ports: day 2, t 18 =6.9, p<0.0001; day 3, t 18 =8.4, p<0.0001). Flpo-/-control mice (Flp-; n=9) nose poked at equal amounts across both ports (Two-Way ANOVA, main effect of port-type: F 1,8 =2.7, p=0.14). (F) Flp+ mice showed a higher percent preference for active port than Flp-mice (Two-Way ANOVA, main effect of group: F 1,17 =8.3, p=0.01) that grew over days (day x group interaction: F 2,34 =4.5, p=0.02; Bonferroni multiple comparisons between Flp+ and Flp-preference on day 3: t 13 =3.9, p<0.005). (G) Strategy to express ChR2 and a CRISPR-Cas9 AAV targeted to targeted to the control ROSA26 locus (sgROSA), or to disrupt expression of VGAT (sgVGAT) or VGLUT2 (sgVGAT) in VTA GG neurons; plus optic fibers implanted bilaterally above LHb. (H) Active vs. inactive nose pokes in self-stimulation assay (same as depicted in panel D) for all four groups. Flp-/-control mice (Flp-; n=11) poked equally between ports (Two-Way ANOVA, main effect of port-type: F 1,10 =2.7, p=0.16), sgRosa26 control mice preferred the active port (n=9): Two-Way ANOVA, main effect of port-type: F 1,8 =13, p=0.007; day x port interaction: F 2,16 =0.8, p=0.47; Bonferroni multiple comparisons between active vs. inactive ports: day 1, t 16 =7.3, p<0.0001; day 2, t 16 =5.7, p<0.0001; day 3, t 16 =5.8, p<0.0001); sgVGLUT2 mice preferred the active port (n=9): Two-Way ANOVA, main effect of port-type: F 1,8 =12.4, p=0.008; day x port interaction: F 2,16 =5.7, p=0.01; Bonferroni multiple comparisons between active vs. inactive ports: day 1, t 16 =0.95, p>0.99; day 2, t 16 =3.5, p<0.009; day 3, t 16 =5.7, p<0.0001); sgVGAT mice showed no preference for active port (n=11): Two-Way ANOVA, main effect of port-type: F 1,10 =3.5, p=0.09; day x port interaction: F 2,20 =2.4, p=0.11). Data are shown as mean +/-SEM; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: bioRxiv

    Article Title: GABA-glutamate corelease is a mechanism for state-dependent neurotransmission

    doi: 10.1101/2025.11.19.689286

    Figure Lengend Snippet: The reinforcing effects of VTA GG input to LHb require GABA release. (A) Strategy to express ChR2:eYFP in VTA GG neurons, plus optic fibers implanted bilaterally above LHb. (B) Terminal expression of ChR2 in LHb of VGLUT2-Cre; VGAT-Flp mice and (C) control mouse lacking Flp; scale = 500 um. (D) Schematic of optogenetic self-stimulation assay. Nose pokes into the active port delivered 40 Hz 1s blue (473 nm) light plus a 1s tone, while nose pokes into the inactive port delivered a 1s tone alone. (E) ChR2-expressing mice (Flp+; n=10) nose poked at greater amounts in the active port compared to the inactive port (Two-Way ANOVA, main effect of port-type: F 1,9 =29, p=0.0004; day x port interaction: F 2,18 =9.3, p=0.002; Bonferroni multiple comparisons between active vs. inactive ports: day 2, t 18 =6.9, p<0.0001; day 3, t 18 =8.4, p<0.0001). Flpo-/-control mice (Flp-; n=9) nose poked at equal amounts across both ports (Two-Way ANOVA, main effect of port-type: F 1,8 =2.7, p=0.14). (F) Flp+ mice showed a higher percent preference for active port than Flp-mice (Two-Way ANOVA, main effect of group: F 1,17 =8.3, p=0.01) that grew over days (day x group interaction: F 2,34 =4.5, p=0.02; Bonferroni multiple comparisons between Flp+ and Flp-preference on day 3: t 13 =3.9, p<0.005). (G) Strategy to express ChR2 and a CRISPR-Cas9 AAV targeted to targeted to the control ROSA26 locus (sgROSA), or to disrupt expression of VGAT (sgVGAT) or VGLUT2 (sgVGAT) in VTA GG neurons; plus optic fibers implanted bilaterally above LHb. (H) Active vs. inactive nose pokes in self-stimulation assay (same as depicted in panel D) for all four groups. Flp-/-control mice (Flp-; n=11) poked equally between ports (Two-Way ANOVA, main effect of port-type: F 1,10 =2.7, p=0.16), sgRosa26 control mice preferred the active port (n=9): Two-Way ANOVA, main effect of port-type: F 1,8 =13, p=0.007; day x port interaction: F 2,16 =0.8, p=0.47; Bonferroni multiple comparisons between active vs. inactive ports: day 1, t 16 =7.3, p<0.0001; day 2, t 16 =5.7, p<0.0001; day 3, t 16 =5.8, p<0.0001); sgVGLUT2 mice preferred the active port (n=9): Two-Way ANOVA, main effect of port-type: F 1,8 =12.4, p=0.008; day x port interaction: F 2,16 =5.7, p=0.01; Bonferroni multiple comparisons between active vs. inactive ports: day 1, t 16 =0.95, p>0.99; day 2, t 16 =3.5, p<0.009; day 3, t 16 =5.7, p<0.0001); sgVGAT mice showed no preference for active port (n=11): Two-Way ANOVA, main effect of port-type: F 1,10 =3.5, p=0.09; day x port interaction: F 2,20 =2.4, p=0.11). Data are shown as mean +/-SEM; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Article Snippet: Slc17a6 +/Cre (VGLUT2-IRES-cre) mice and Slc32a1 Flp+/- (VGAT-Flp) mice were initially obtained from Jackson Laboratory (Stocks: 018147 and 029591, respectively), and were crossed to generate a dual transgenic, VGLUT2-cre/VGAT-Flp line.

    Techniques: Expressing, Control, CRISPR